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s aureus parental strain atcc 29213  (ATCC)


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    Structured Review

    ATCC s aureus parental strain atcc 29213
    S Aureus Parental Strain Atcc 29213, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+aureus+parental+strain+atcc+29213/Staphylococcus+aureus%3B+subsp%2E+aureus/us11129884-554-7-11
    Average 99 stars, based on 9738 article reviews
    s aureus parental strain atcc 29213 - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: The expression of a putative exotoxin and an ABC transporter during bovine intramammary infection contributes to the virulence of Staphylococcus aureus.
    Article Snippet: Staphylococcus aureus is a leading cause of intramammary infections (IMI) and bovine mastitis is an important disease for the dairy industry.. As this bacterium probably expresses specific genes for establishment of IMI, we studied the transcriptional profile of four S. aureus strains recovered from experimentally infected cows.. Microbial RNA was extracted from bacteria isolated from milk, reverse-transcribed and labeled for hybridization to sub-genomic microarrays to detect candidate genes for further investigations.

    Transformation Assay:

    Article Title: The expression of a putative exotoxin and an ABC transporter during bovine intramammary infection contributes to the virulence of Staphylococcus aureus.
    Article Snippet: Staphylococcus aureus is a leading cause of intramammary infections (IMI) and bovine mastitis is an important disease for the dairy industry.. As this bacterium probably expresses specific genes for establishment of IMI, we studied the transcriptional profile of four S. aureus strains recovered from experimentally infected cows.. Microbial RNA was extracted from bacteria isolated from milk, reverse-transcribed and labeled for hybridization to sub-genomic microarrays to detect candidate genes for further investigations.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Gene SACOL COL N315 RF122 USA300 MSSA476 0442 enterotoxin, similar to hypothetical enterotoxin, putative putative exotoxin 2 protein putative exported protein 0718- ABC ABC ABC ABC putative ABC 0720 transporter, transporter, transporter, transporter, transporter ATP-binding ATP-binding ATP-binding ATP-binding protein and protein and protein and protein and protein and permease permease permease permease permease 2365 lipoprotein, hypothetical lipoprotein, lipoprotein, lipoprotein, putative protein, similar putative putative putative to TpgX protein 0029 HMG-CoA probable — conserved — synthase, HMG-CoA hypothetical truncation synthase protein 1416 peptide ABC oligopeptide probable peptide ABC putative transporter, transporter oligopeptide transporter, oligopeptide permease membrane membrane permease transport system protein, permease permease protein permease putative domain (opp2c) 1944 conserved conserved conserved conserved putative hypothetical hypothetical hypothetical hypothetical membrane protein protein protein protein protein 1611 transcriptional ferric uptake zinc-specific ferric uptake zinc-specific regulator, Fur regulator metalloregulator regulation metalloregulatory family homolog (zur) protein (fur) protein 2599 conserved hypothetical probable transporter putative domain protein protein, similar membrane gate domain membrane to ferrous iron protein protein protein transporter 2144 ABC ABC ABC ABC ABC transporter, transporter, transporter, transporter, transporter, ATP-binding ATP-binding ATP-binding ATP-binding ATP-binding protein protein protein protein protein 1353 ABC hypothetical — ABC putative transporter, protein, similar transporter, membrane permease to ABC permease protein protein, transporter protein putative integral 0264 ABC conserved probable ABC ABC putative ABC transporter, hypothetical transporter ATP transporter, transporter ATP- ATP-binding protein binding protein ATP-binding binding protein protein protein Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al, 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components from
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et at, 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Electroporation:

    Article Title: The expression of a putative exotoxin and an ABC transporter during bovine intramammary infection contributes to the virulence of Staphylococcus aureus.
    Article Snippet: Staphylococcus aureus is a leading cause of intramammary infections (IMI) and bovine mastitis is an important disease for the dairy industry.. As this bacterium probably expresses specific genes for establishment of IMI, we studied the transcriptional profile of four S. aureus strains recovered from experimentally infected cows.. Microbial RNA was extracted from bacteria isolated from milk, reverse-transcribed and labeled for hybridization to sub-genomic microarrays to detect candidate genes for further investigations.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Gene SACOL COL N315 RF122 USA300 MSSA476 0442 enterotoxin, similar to hypothetical enterotoxin, putative putative exotoxin 2 protein putative exported protein 0718- ABC ABC ABC ABC putative ABC 0720 transporter, transporter, transporter, transporter, transporter ATP-binding ATP-binding ATP-binding ATP-binding protein and protein and protein and protein and protein and permease permease permease permease permease 2365 lipoprotein, hypothetical lipoprotein, lipoprotein, lipoprotein, putative protein, similar putative putative putative to TpgX protein 0029 HMG-CoA probable — conserved — synthase, HMG-CoA hypothetical truncation synthase protein 1416 peptide ABC oligopeptide probable peptide ABC putative transporter, transporter oligopeptide transporter, oligopeptide permease membrane membrane permease transport system protein, permease permease protein permease putative domain (opp2c) 1944 conserved conserved conserved conserved putative hypothetical hypothetical hypothetical hypothetical membrane protein protein protein protein protein 1611 transcriptional ferric uptake zinc-specific ferric uptake zinc-specific regulator, Fur regulator metalloregulator regulation metalloregulatory family homolog (zur) protein (fur) protein 2599 conserved hypothetical probable transporter putative domain protein protein, similar membrane gate domain membrane to ferrous iron protein protein protein transporter 2144 ABC ABC ABC ABC ABC transporter, transporter, transporter, transporter, transporter, ATP-binding ATP-binding ATP-binding ATP-binding ATP-binding protein protein protein protein protein 1353 ABC hypothetical — ABC putative transporter, protein, similar transporter, membrane permease to ABC permease protein protein, transporter protein putative integral 0264 ABC conserved probable ABC ABC putative ABC transporter, hypothetical transporter ATP transporter, transporter ATP- ATP-binding protein binding protein ATP-binding binding protein protein protein Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al, 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components from
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et at, 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.

    Article Title: Bacterial vaccine components and uses thereof
    Article Snippet: Mutants for genes SACOL0442 and SACOL0720 were produced by gene replacement for mutant Δ442a, (Mitchell et al., 2008) and by intron insertion for mutants Δ442b and Δ720 (TargeTron Gene Knockout System, Sigma Aldrich (Chen et al., 2007)). .. The mutants were carried out in the S. aureus parental strain ATCC 29213 that could be easily transformed by electroporation. .. For creating mutant Δ442a, a 223-pb fragment of gene SACOL0442 in strain ATCC 29213 was deleted and replaced by insertion of the 1300-bp erythromycin resistance gene ermA between positions 188 and 411 of the nucleotide sequence of SACOL0442.



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